• Question: Have you used chromatography?

    Asked by anon-361056 on 28 Mar 2023.
    • Photo: Martin McCoustra

      Martin McCoustra answered on 28 Mar 2023:

      I don’t use it personally in my research but I work with colleagues who do and are expert at using gas chromatography coupled to mass spectrometry to study volatile flavour compounds in food and drink.

    • Photo: Graeme Dykes

      Graeme Dykes answered on 30 Mar 2023:

      Lots and lots and lots of chromatography.
      I have lost count of the times that I have run thin-layer chromatography to monitor reactions.
      Huge numbers of silica based flash column chromatography to purify compounds.
      It has been a while since I used Gas chromatography (GC) but High Pressure Liquid Chromatography (HPLC), is a great tool for checking purity, especially when combined with mass spectrometry (LCMS).
      Preparative LCMS is a good way to purify lots of molecules.
      Affinity chromatography can be a big deal in biology but even in chemistry, it can speed up purification.
      Most recently, I have been using size exclusion chromatography!

    • Photo: Jade Markham

      Jade Markham answered on 31 Mar 2023:

      Only at university to learn about chromatography and how to use it.

      I liked making chemicals more than analysising them

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